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Genome Editor
Genome Editor
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High throughput and effcient CRISPR/Cas9-based mouse genome editing by RNA electroporation to zygote


Fgf10 gene knockout mouse by Cas9 mRNA/gRNA electroporation
Fgf10 homozygous mutant embryo have an easily detectable limbless phonotype. Electroporation of Cas9 mRNA (Cas9 Protein) and gRNA enables genome editing of Fgf10.


Electrodes for zygote genome editing

Platinum plate electrode on tempered glass, LF501PT1-10, can hold up to ~40 mouse zygotes in a 5 μL electroporation buffer and enables efficient and high throughput zygote genome editing using CRISPR/Cas9 by electroporation.